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rabbit polyclonal anti-dopamine receptor d1 (drd1 (Santa Cruz Biotechnology)

Name: rabbit polyclonal anti-dopamine receptor d1 (drd1
Brand: Santa Cruz Biotechnology
Rating: 90 (1 reviews)

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Santa Cruz Biotechnology rabbit polyclonal anti-dopamine receptor d1 (drd1

HF, Hizikia fusiformis extract treatment; VEH, vehicle treatment (control); <t>DRD1-5,</t> dopamine <t>D1–5</t> receptors; DAT, dopamine transporter; DAPI, 4′,6-diamidino-2-phenylindole; NF-L, neurofilament light chain; SERT, serotonin transporter; TH, tyrosine hydroxylase; TPH, tryptophan hydroxylase; SYP, synaptophysin; 5HT1A and 1B, serotonin 1A and 1B receptors; MAP2, microtubule-associated protein 2.

Rabbit Polyclonal Anti Dopamine Receptor D1 (Drd1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti-dopamine receptor d1 (drd1/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews

rabbit polyclonal anti-dopamine receptor d1 (drd1 - by Bioz Stars, 2026-02

90/100 stars

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1) Product Images from "Dopamine and serotonin alterations by Hizikia fusiformis extracts under in vitro cortical primary neuronal cell cultures"

Article Title: Dopamine and serotonin alterations by Hizikia fusiformis extracts under in vitro cortical primary neuronal cell cultures

Journal: Nutrition Research and Practice

doi: 10.4162/nrp.2023.17.3.408

HF, Hizikia fusiformis extract treatment; VEH, vehicle treatment (control); DRD1-5, dopamine D1–5 receptors; DAT, dopamine transporter; DAPI, 4′,6-diamidino-2-phenylindole; NF-L, neurofilament light chain; SERT, serotonin transporter; TH, tyrosine hydroxylase; TPH, tryptophan hydroxylase; SYP, synaptophysin; 5HT1A and 1B, serotonin 1A and 1B receptors; MAP2, microtubule-associated protein 2.

Figure Legend Snippet: HF, Hizikia fusiformis extract treatment; VEH, vehicle treatment (control); DRD1-5, dopamine D1–5 receptors; DAT, dopamine transporter; DAPI, 4′,6-diamidino-2-phenylindole; NF-L, neurofilament light chain; SERT, serotonin transporter; TH, tyrosine hydroxylase; TPH, tryptophan hydroxylase; SYP, synaptophysin; 5HT1A and 1B, serotonin 1A and 1B receptors; MAP2, microtubule-associated protein 2.

Techniques Used:

DRD1–5, dopamine D1–5 receptors; HF, Hizikia fusiformis extract treatment; VEH, vehicle treatment (control); DAT, dopamine transporter; TH, tyrosine hydroxylase.

Figure Legend Snippet: DRD1–5, dopamine D1–5 receptors; HF, Hizikia fusiformis extract treatment; VEH, vehicle treatment (control); DAT, dopamine transporter; TH, tyrosine hydroxylase.

Techniques Used:

Figure Legend Snippet: A summary of BF 10 with post-hoc pair wise comparisons

Techniques Used:

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Journal: Nutrition Research and Practice

Article Title: Dopamine and serotonin alterations by Hizikia fusiformis extracts under in vitro cortical primary neuronal cell cultures

doi: 10.4162/nrp.2023.17.3.408

Figure Lengend Snippet: HF, Hizikia fusiformis extract treatment; VEH, vehicle treatment (control); DRD1-5, dopamine D1–5 receptors; DAT, dopamine transporter; DAPI, 4′,6-diamidino-2-phenylindole; NF-L, neurofilament light chain; SERT, serotonin transporter; TH, tyrosine hydroxylase; TPH, tryptophan hydroxylase; SYP, synaptophysin; 5HT1A and 1B, serotonin 1A and 1B receptors; MAP2, microtubule-associated protein 2.

Article Snippet: In this study, we used primary antibodies for DA transmission: mouse monoclonal anti-tyrosine hydroxylase (TH; Abcam; catalog #ab129991), rabbit polyclonal anti-DAT (Santa Cruz; catalog #sc-14002), rabbit polyclonal anti-dopamine receptor D1 (DRD1; Santa Cruz; catalog #sc-14001), mouse monoclonal anti-DRD2 (Santa Cruz; catalog #sc-5303), mouse monoclonal anti-DRD3 (Santa Cruz; catalog #sc-136170), rabbit polyclonal anti-DRD4 (Elabscience; catalog #E-AB-31153), and rabbit polyclonal anti-DRD5 (Mybiosource; catalog #MBS2516950) antibodies; those for 5-HT transmission: rabbit polyclonal anti-5HT1A (Elabscience; catalog #E-AB-32950), rabbit polyclonal anti-5HT1B (Abcam; catalog #ab13896), anti-tryptophan hydroxylase (TPH; catalog #E-AB-16937), and mouse monoclonal anti-SERT (Santa Cruz; catalog #sc-33724) antibodies; and those for neuronal structures: mouse monoclonal anti-synaptophysin (SYP; Sigma-Aldrich; catalog #S5768), mouse monoclonal anti-microtubule-associated protein 2 (MAP2; Santa Cruz; catalog #sc-32791), and mouse monoclonal neurofilament-light chain (NF-L; Santa Cruz; catalog #sc-20012) antibodies.

Techniques:

Journal: Nutrition Research and Practice

Article Title: Dopamine and serotonin alterations by Hizikia fusiformis extracts under in vitro cortical primary neuronal cell cultures

doi: 10.4162/nrp.2023.17.3.408

Figure Lengend Snippet: DRD1–5, dopamine D1–5 receptors; HF, Hizikia fusiformis extract treatment; VEH, vehicle treatment (control); DAT, dopamine transporter; TH, tyrosine hydroxylase.

Techniques:

Journal: Nutrition Research and Practice

Article Title: Dopamine and serotonin alterations by Hizikia fusiformis extracts under in vitro cortical primary neuronal cell cultures

doi: 10.4162/nrp.2023.17.3.408

Figure Lengend Snippet: A summary of BF 10 with post-hoc pair wise comparisons

Techniques:

RNA sequencing data analysis in nax ( n = 5) and wild–type (wt) ( n = 6) mice. Data analysis shows dopamine receptor D1 ( Drd1, A ), Drd3 ( C ), and Drd4 ( D ) are significantly increased in the nax cerebellum. Although an increase in Drd2 ( B ) and Drd5 ( E ) is apparent, statistical significance was not reached. The data in the bar graph are presented as the mean ± SEM, and statistical analysis was performed using an unpaired t –test (* p < 0.05 and ** p < 0.01). RPKM: Reads Per Kilobase of transcript per Million mapped reads.

Journal: International Journal of Molecular Sciences

Article Title: Alteration of the Dopamine Receptors’ Expression in the Cerebellum of the Lysosomal Acid Phosphatase 2 Mutant (Naked–Ataxia ( NAX )) Mouse

doi: 10.3390/ijms21082914

Figure Lengend Snippet: RNA sequencing data analysis in nax ( n = 5) and wild–type (wt) ( n = 6) mice. Data analysis shows dopamine receptor D1 ( Drd1, A ), Drd3 ( C ), and Drd4 ( D ) are significantly increased in the nax cerebellum. Although an increase in Drd2 ( B ) and Drd5 ( E ) is apparent, statistical significance was not reached. The data in the bar graph are presented as the mean ± SEM, and statistical analysis was performed using an unpaired t –test (* p < 0.05 and ** p < 0.01). RPKM: Reads Per Kilobase of transcript per Million mapped reads.

Article Snippet: D1: rabbit polyclonal anti–D1 dopamine receptor (TA328798, anti–Drd1, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA); D2: rabbit polyclonal anti–D2 dopamine receptor (TA328800, anti–Drd2, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 2 (DR2); D3: rabbit polyclonal anti–D3 dopamine receptor (TA328800, anti–Drd3, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 3 (DR3); D4: rabbit polyclonal anti–D4 dopamine receptor (TA321202, anti–DRD4, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor D4 (DRD4); and D5: rabbit polyclonal anti–D5 dopamine receptor (TA328802, anti–Drd5, diluted 1:1000; OriGene Biotech Co., Rockville, MD, USA), produced against recombinant rat dopamine receptor 5 (DR5).

Techniques: RNA Sequencing Assay

Frontal sections of wt and nax mouse cerebella: DRD1 expression at P5 and P17. ( A ) Immunoperoxidase staining for DRD1 in the wt cerebellum on P5 shows the immunoreactivity in the Purkinje cell layer (Pcl) and white matter (wm) but not in the external germinal zone (egz) and granular layer (gl). ( B ) Immunoperoxidase staining for DRD1 in the nax cerebellum shows similar immunoreactivity as in the wt littermates at P5. ( C ) DRD1 immunostaining of the frontal sections of the wt cerebellum at P17 shows immunoreactivity in the Purkinje cell (PC) somata, the Pcl, and dendrites in the molecular layer (ml) and in the gl but not in the wm. ( D ) DRD1 immunostaining of the frontal sections of the nax cerebellum on P17 shows immunoreactivity in the PCs in the ml/Pcl. ( E ) Western blot analysis of whole cerebellar lysates revealed no significant differences in DRD1 expression between wt and nax cerebella at P5 and P17 (wt: n = 3 and nax : n = 3). The data in the bar graphs are presented as the means of three independent experiments ± SEM, and statistical analysis was performed using a two–way ANOVA. P; postnatal. Scale bars: 100 μm in A, B, C, and D.

Journal: International Journal of Molecular Sciences

Article Title: Alteration of the Dopamine Receptors’ Expression in the Cerebellum of the Lysosomal Acid Phosphatase 2 Mutant (Naked–Ataxia ( NAX )) Mouse

doi: 10.3390/ijms21082914

Figure Lengend Snippet: Frontal sections of wt and nax mouse cerebella: DRD1 expression at P5 and P17. ( A ) Immunoperoxidase staining for DRD1 in the wt cerebellum on P5 shows the immunoreactivity in the Purkinje cell layer (Pcl) and white matter (wm) but not in the external germinal zone (egz) and granular layer (gl). ( B ) Immunoperoxidase staining for DRD1 in the nax cerebellum shows similar immunoreactivity as in the wt littermates at P5. ( C ) DRD1 immunostaining of the frontal sections of the wt cerebellum at P17 shows immunoreactivity in the Purkinje cell (PC) somata, the Pcl, and dendrites in the molecular layer (ml) and in the gl but not in the wm. ( D ) DRD1 immunostaining of the frontal sections of the nax cerebellum on P17 shows immunoreactivity in the PCs in the ml/Pcl. ( E ) Western blot analysis of whole cerebellar lysates revealed no significant differences in DRD1 expression between wt and nax cerebella at P5 and P17 (wt: n = 3 and nax : n = 3). The data in the bar graphs are presented as the means of three independent experiments ± SEM, and statistical analysis was performed using a two–way ANOVA. P; postnatal. Scale bars: 100 μm in A, B, C, and D.

Techniques: Expressing, Immunoperoxidase Staining, Immunostaining, Western Blot

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